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Human IL-3
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商品描述
Human IL-3，130-093-909，MiltenyiBiotec:
overview：
Details:
overview：
IL-3 stands for interleukin 3. Human IL-3 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Applications
Human IL-3 can be used for a variety of applications, including:
- Induction of colony formation from hematopoietic progenitor cells in semi-solid medium in vitro , for example, CD34+ cells from umbilical cord blood2 .
- In vitro differentiation studies, for example, of B lymphoid progenitors3 .
- Cultivation of plasmacytoid dendritic cells4 .
- In vitro expansion of hematopoietic stem cells.
- Investigation of mast cell or basophil function, for example, basophil interaction with blood vessels5 .
- Investigation of IL-3–mediated signaling pathways.
Details:
Background information
Interleukin 3 (IL-3) is a hematopoietic growth factor, which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biological activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.
Quality description
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request ([email protected]).
Biological activity
-
Proliferation of TF-1 cells (NIBSC 91/510)
-
premium grade: ≥ 2×
10
6
IU/mg
(typical activity: ≥ 2.3×
10
6
IU/mg
)
-
research grade: ≥ 1×
10
6
IU/mg
Interleukin 3 (IL-3) is a hematopoietic growth factor, which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biological activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request ([email protected]).
Proliferation of TF-1 cells (NIBSC 91/510)
premium grade: ≥ 2×
10
6
IU/mg
(typical activity: ≥ 2.3×
)
10
6
IU/mg
research grade: ≥ 1×
10
6
IU/mg
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100T
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1kit
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autoMACS
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1500ml
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autoMACS
Rinsing Solution
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6×1.45L
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24
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200-074-401
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