Anti-Histone H1,H126,Leinco
Clone: AE4
Product Type: Monoclonal Antibody
Isotype: Mouse IgG2a
Applications: IHC (Frozen), WB
|
Prod No.
|
Size
|
Price
|
Avail.
|
Qty
|
Add to cart
|
|
|
|
|
|
|
|
Antibody Details
Formulation
Anti-Human Histone H1 is supplied in filtered tissue supernatant (RPMI-1640) pH-7.2 containig 0.09% sodium azide.
Storage and Handling
For long term storage freeze working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 μg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 10 μg/ml.
Description
Specificity
Anti-Human Histone H1 (Clone AE4) recognizes an antigen associated with Human Histone H1 in the nucleaus of animals and plants. The sequence of the epitope is unmapped.
Background
Histone H1 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. A variant of the histone H1 protein is the histone H5, which has a similar structure and function, but is only found in avian erythrocytes, which unlike mammals have nuclei. Featuring a central globular domain and long C and N terminal tails H1 is involved with the packing of the 'beads on a string' structure into the '30nm solenoid' structure. H1 is present in half the amount of the other four histones. This is because unlike the other histones, H1 does not make up the nucleosome 'bead'. Instead, it sits on top of the structure, keeping in place the DNA that has wrapped around the nucleosome. Specifically, the H1 protein binds to the linker DNA (approximately 80 nucleotides in length) region between the histone beads, helping stabilize the zig-zagged 30nm chromatin fiber. H1 histone seals off the nucleosome at the location at which the linker DNA enters and leaves.
Antigen Details
References & Citations
1. Oku, N. J et al. (2001) Cell Sci.114(Pt 9):1631-41. Article Link
2. Yoshida, K. et al. (1997) FEBS Lett. 401(2-3):247-51. Article Link
3. Yoshida, K. et al. (1997) J Biol Chem. 272(26):16657-62. Article Link
4. Yoshida, K. and Mizukami, Y. (1997) Biochem J. 323 (Pt 3):785-90. PubMed
Technical Protocols
