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Product name

Anti-Laminin antibody
See all Laminin primary antibodies

Description

Rabbit polyclonal to Laminin

Host species

Rabbit

Specificity

In dot blot immunoassay this antibody does not react with Fibronectin, Vitronectin, Collagen IV, or Chondroitin sulfate types A, B, and C.

Tested applications

Suitable for: IHC-FoFr,IHC-Fr,WB,IP,IHC - Wholemount,Dot blot,IHC-P,ICC/IF

Species reactivity

Reacts with: Mouse, Rat, Horse, Dog, Human, Pig, Xenopus laevis
Predicted to work with: Reptile, Mammals, Amphibian

Immunogen

Full length native protein (purified) corresponding to Laminin. The immunogen is laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.

Positive control

• IHC-P: Human skin tissue; mouse skin neoplasia tissue. IHC-Fr: Mouse skin, placenta, anterior tibialis skeletal muscle and testis tissue.

General notes

Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor^? 488 (ab150077).

See other anti-rabbit secondary antibodies that can be used with this antibody.

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Cellular and embryonic localisation of CL-L1

Immunofluorescence showing co-localisation of CL-L1 and Laminin in E13.5 mouse embryos sections. CL-L1 is expressed in the basal membrane of the ephithelium in the palate shelf of the maxilla (arrows). In contrast Laminin expression is present all around the ephitelium membrane. A faint but clear CL-L1 expression is also observed in the cytoplasm of the epithelium and in the mesenchyme of the palate. PSM; rostral extremity of right palatal shelf of maxilla. Scale bar 100 μm.

(After Fugure 2 E of Munye et al)

This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion, anti-laminin 1:200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.

ab11575 staining Laminin (red) in mouse postnatal day 14 testes tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and blocked with 0.3% Triton X-100 + 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. A TRITC-conjugated donkey anti-rabbit IgG (H+L) polyclonal (1/200) was used as the secondary antibody. Green - HSD3V1-FITC.

ab11575 staining Laminin in mouse anterior tibialis skeletal muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was permeabilized with 0.5% Triton X in PBS for 5 minutes. Samples were incubated with primary antibody (1/1000 in PBS) for 30 minutes at 25°C. An Alexa Fluor^? 488-conjugated goat anti-rabbit IgG polyclonal (1/600) was used as the secondary antibody.

ab11575 at a 1/200 dilution staining mouse placenta tissue sections by Immunohistochemistry (frozen sections). The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 12 hours. Bound antibody was detected using an Alexa Fluor ? 594 conjugated goat anti-rabbit IgG.

ab11575 staining Laminin in mouse oocytes by Immunohistochemistry (PFA fixed, paraffin embedded sections).
Grafts were fixed overnight in 4% paraformaldehyde, embedded in paraffin, and sectioned in 5 to 8 μm intervals. In brief, sections on slides were de-paraffinized, re-hydrated, antigens unmasked by incubating in target retrieval solution at 95°C for 30 minutes, permeabilized in 0.1% Triton-X100 for 5 minutes, blocked with 10% chicken serum in TBST overnight, and incubated with primary antibody at 1/200, in TBST with 1% serum for 1 hour at room temperature. After washing in TBST, slides were incubated with secondary antibody at 1/1000, for 30 minutes at room temperature. Cover slips were mounted with Prolong Gold Antifade with DAPI.

Immunofluorescence revealed TRA98+ oocytes and FOXL2+ granulosa cells in ovarian cord-like structures (dashed lines) and Laminin+ basement membrane (red) following five days of intact e12.5 female genital ridge transplantation. Alo

ab11575 staining Laminin in Mouse skin neoplasia - papilloma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/200) for 8 hours at 4°C. A biotin-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

ab11575 staining Laminin in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone, permeabilized with PBST (Triton X 100 0.025%) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/250) for 1 hour. An undiluted Alexa Fluor^?488-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody. The image shows 2 differeent skin sections with Laminin (green) staining in the basal layer of the epidermis. DAPI staining in blue.