ELISA Kit

人胎盤(pán)堿性磷酸酶(PLAP)ELISA Kit

Human Placental alkaline phosphatase,PLAP ELISA Kit

CSB-E09160h

ALP, FLJ61142, PALP, PLAP, alkaline phosphomonoesterase|glycerophosphatase|placental alkaline phosphatase

96T

3200

Homo sapiens (Human)

alkaline phosphatase, placental (Regan isozyme)

ALPP

84 pg/mL-2000 pg/mL

34 pg/mL

1-5h

50-100ul

450 nm

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   2000 2.012 1.983 1.998 1.952   1000 1.338 1.341 1.340 1.294   500 0.775 0.828 0.802 0.756   167 0.357 0.349 0.353 0.307   84 0.224 0.235 0.230 0.184   0 0.047 0.045 0.046

Intra-assay Precision (Precision within an assay): CV%<10%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of human PLAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		85-98
1:2		Average %		96
		Range %		88-100
1:4		Average %		95
		Range %		89-100
1:8		Average %		97
		Range %		91-101

The recovery of human PLAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		93-104
EDTA plasma (n=4)		95		91-101

quantitative

Tags & Cell Markers