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Human Paxillin Antibody

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商品描述

Human Paxillin Antibody ，MAB4259-SP,R&D：

Human Paxillin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Paxillin in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 492918

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Paxillin
Asn59-Ser274
Accession # P49023

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 μg/mL

See below

Immunocytochemistry

8-25 μg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Paxillin by Western Blot.

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Detection of Human Paxillin by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 μg/mL of Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific diffuse band was detected for Paxillin at approximately 65 to 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry Paxillin in Detroit 551 Human Cell Line.

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Paxillin in Detroit 551 Human Cell Line. Paxillin was detected in immersion fixed Detroit 551 human embryonic skin fibroblast cell line using Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) at 10 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Paxillin in HUVEC Human Cells.

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Paxillin in HUVEC Human Cells. Paxillin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Paxillin Monoclonal Antibody (Catalog # MAB4259) at 10 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to actin filament tips. View our protocol for Fluorescent ICC Staining of Cells on Coverslips

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Human Paxillin Antibody

Human Paxillin Antibody ，MAB4259-SP,R&D：

Human Paxillin Antibody Summary

Applications

Data Examples

Human Paxillin Antibody ，MAB4259-SP,R&D：